Disclosed is a novel Lepidopteran- and Coleopteran-active
.delta.-endotoxin polypeptide, and compositions comprising the
polypeptide, peptide fragments thereof, and antibodies specific therefor.
Also disclosed are vectors, transformed host cells, and transgenic plants
that comprise nucleic acid segments encoding the polypeptide. Also
disclosed are methods of identifying related polypeptides and
polynucleotides, methods of making and using transgenic cells comprising
the novel sequences of the invention, as well as methods for controlling
an insect population, such as the Western Corn Rootworm and Colorado
potato beetle, and for conferring to a plant population resistance to the
target insect species.