The invention provides a method to regulate expression of a gene of
interest in a plant comprising, introducing into the plant a first
nucleotide sequence comprising, the gene of interest operatively linked
to a first regulatory region, and an operator sequence capable of binding
a fusion protein, and a second nucleotide sequence comprising a second
regulatory region in operative association with a nucleotide sequence
encoding the fusion protein. The fusion protein comprising, a DNA binding
protein, or a portion thereof, capable of binding the operator sequence,
and a recruitment factor protein, or a portion thereof, capable of
binding a chromatin remodelling protein. In this manner, expression of
the second nucleotide sequence produces the fusion protein that regulates
expression of the gene of interest.