Methods are provided for transforming freshly isolated, immature maize
embryos and for producing transgenic maize plants. The methods comprise
obtaining immature embryos from a maize plant, contacting the embryos
with an auxin-depleted or phytohormone-depleted transformation support
medium and introducing a nucleotide construct into cells from the embryos
prior to subjecting the embryos to conditions which promote
embryogenic-tissue formation. The methods additionally comprise
identifying or selecting transformed cells and regenerating such cells
into transformed maize plants.