The device comprises a tube or catheter 1 that is introduced into the animal's vagina up to the cervix duct 19; a flexible probe 6 constituted of a first flexible tubular body 7, to allow that the probe, after reaching the distal end of the tube 1, may progress through the cervix duct 19 and thereafter through the cervix horn 22, this structure allowing to carry out a method of introducing a fluid with spermatozoids, embryos or therapeutic solutions to the anterior third of the uterus horn, or to obtain embryos from the anterior portions of the uterus horn, without needing sedation or anesthesia and without disturbing the animal's well-being, the device, the probe and the method being essentially applied to porcine livestock, small ruminants and any other animal species.