The present invention relates to a genotype-independent method for
transforming and selecting plant explants. The transformation method
includes pre-culturing the explants in the presence of an Agrobacterium
inducer and exposing the transformed explants to a shoot regeneration
media that accelerates shoot development. Plants generated from this
transformation method are provided. In particular, methods for obtaining
transgenic Eucalyptus and pine cells and regenerating stably transformed
Eucalyptus and pine trees are provided. The invention also provides
media, methods, and plasmids for selecting and regenerating plants,
particularly forest trees.