A method of determining an amount of at least one molecular specie
comprising a sample is provided, each molecular specie being indicated by
a dye. The amount of the molecular specie is determined from an image of
the sample captured as image data by a color image acquisition device in
a video-microscopy system. An optical density of the sample is first
determined in each of a red, green, and blue channel at a particular
pixel in the image. A corresponding optical density matrix is thereafter
formed for that pixel. The optical density matrix is then multiplied by
the inverse of a relative absorption coefficient matrix so as to form a
resultant matrix for the pixel. The relative absorption coefficient
matrix comprises a relative absorption coefficient for each dye,
independently of the sample, in each of the red, green, and blue
channels. The resultant matrix thus comprises the amount of each
molecular specie, as indicated by the respective dye, for that pixel.
Associated systems and computer software program products are also
provided.