A cartridge used with a peristaltic pumping apparatus, the cartridge comprising a housing and a retainer. The housing having a front and back portion along with a peripheral surface, where the front side is selectively engageable with the pumping apparatus and has a first channel formed in the front side. The retainer having a front side and a rear side, the front side adapted to receive a length of flexible tubing, where the rear side is receivable in the first channel.