Monocolonal antibodies having a higher reactivity with tartrate-resistant
acid phosphatase 5b (TRACP 5b) than tartrate-resistant acid phosphatase
5a (TRACP 5a) and having a higher specificity to TRACP 5b can be obtained
by cell fusion using as antigens TRACP 5b purified from human
osteoclasts. By using the monoclonal antibody, TRACP 5b in a sample can
be detected specifically with a high sensitivity.