The invention relates a method of regulating the power available at the manipulator of an electronic scalpel to make said manipulator adapted to carry out the cutting operation of an organic tissue, said electronic scalpel being of the kind comprising: at least a rectifying circuit of the mains voltage supplying a rectified and direct voltage to at least a radio frequency circuit adapted to emit an output of a current signal at a substantially constant frequency feeding said manipulator through a radio frequency transformer, said radio frequency circuit comprising at least an electronic switch controlled by a circuit provided with an oscillator, wherein said method consists in applying to the manipulator a wave form having such a power that the energy due to the wave form of the resonance frequency and the harmonics transferred to the manipulator is substantially equal to the sum of the energies required to break the bonds of the molecules of the tissue to be cut. The invention relates also to the electronic scalpel carrying out said method.