The present invention is directed to methods and compositions for
TnpI-mediated genetic engineering using the Bacillus thuringiensis
recombinase TnpI and the TnpI recombination substrates TRT or TRT', and
variants thereof. In particular, the invention relates to TRT or TRT'
sequences, and variants thereof, vectors, cells and kits useful for
TnpI-mediated genetic recombination, as well as methods for the use of
TRT or TRT' sequences for TnpI-mediated genetic engineering.