A method is disclosed for extracting collagen from calcified tissue without a prior decalcification step. The method may be used with calcified tissues such as fish skin with scales, alligator skeletons, and crustacean exoskeletons. It does not require the use of EDTA. When fish skin is used that contains substantial quantities of scales, it is not necessary first to remove scales from the skin before conducting the extraction of collagen.

 
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