cDNA libraries may be obtained from neural cell cultures produced by using
growth factors to induce the proliferation of multipotent neural stem
cells. The libraries may be obtained from both cultured normal and
dysfunctional neural cells and from neural cell cultures at various
stages of development. This information allows for the identification of
the sequence of gene expression during neural development and can be used
to reveal the effects of biological agents on gene expression in neural
cells. Additionally, nucleic acids derived from dysfunctional tissue can
be compared with that of normal tissue to identify genetic material which
may be the cause of the dysfunction. This information could then be used
in the design of therapies to treat the neurological disorder. A further
use of the technology would be in the diagnosis of genetic disorders or
for use in identifying neural cells at a particular stage in development.