There is provided a method for growing human intervertebral cells. Disc
tissue is surgically removed from a normal disc of a patient, the cells
expanded by feeding with a cell stimulant such as a growth factor, or a
cytokine or a bioactive agent to form monolayer primary cell cultures on
a plastic mesh such as a nylon mesh. In the case of a growth factor,
fetal bovine serum is preferred as it improves cell proliferation and
production of appropriate extracellular matrix components. In another
aspect of this invention, the monolayer primary cell cultures are seeded
in alginate or agarose and fed again with the cell stimulant until
three-dimensional cell cultures are formed. The cells are recovered from
the alginate or agarose or from monolayer cultures. Re-implantation is
carried out using bioresorbable carriers or cell suspensions.