The present invention provides an advance in phage display technology by
permitting the uncoupling of the propagation of phages containing
inserted sequences encoding heterologous polypeptides from the expression
of said polypeptides. The invention provides phage constructs and methods
for their use to permit phage coat protein expression, and thus phage
propagation, in the absence of display of heterologous polypeptides,
which may be expressed as a fusion with said coat protein in a regulated
manner.