Methods for amplification and sequencing of at least one nucleic acid
comprising the following steps: (1) forming at least one nucleic acid
template comprising the nucleic acid(s) to be amplified or sequenced,
wherein said nucleic acid(s) to be amplified or sequenced, wherein said
nucleic acid(s) contains at the 5' end an oligonucleotide sequence Y and
at the 3' end an oligonucleotide sequence Z and, in addition, the nucleic
acid(s) carry at the 5' end a means for attaching the nucleic acid(s) to
a solid support; (2) mixing said nucleic acid template(s) with one or
more colony primers X, which can hybridize to the oligonucleotide
sequence Z and carries at the 5' end a means for attaching the colony
primers to a solid support, in the presence of a solid support so that
the 5' ends of both the nucleic acid template and the colony primers bind
to the solid support; (3) performing one or more nucleic acid
amplification reactions on the bound template(s), so that nucleic acid
colonies are generated and optionally, performing at least one step of
sequence determination of one or more of the nucleic acid colonies
generated. Solid supports, kits and apparatus for use in these methods.