A multi-purpose paper, a manufacturing method thereof, and an application thereof are provided. The method of manufacturing the multi-purpose paper comprises steps of (a) providing a papermaking material and obtaining a clean paper pulp by treating the papermaking material, (b) adding at least one functional additive into the clean paper pulp to form the multi-purpose paper, and (c) processing the multi-purpose paper to form thereon an uneven surface structure. The cultivating paper has the combined functions of weed control, pest control, supplying the required nutrients to plants to help develop the root system, maintaining moisture and breath for the root system. The multi-purpose paper can be carriers of agricultural antagonistic microorganisms and serve as the basic functional substrate for weed control, pest control, sowing, growing seedlings, fertilization, fertilizer saving, manpower saving and organic cultivating.