Novel products comprising conditioned cell culture medium compositions and
methods of use are described. The conditioned cell medium compositions of
the invention may be comprised of any known defined or undefined medium
and may be conditioned using any eukaryotic cell type. The medium may be
conditioned by stromal cells, parenchymal cells, mesenchymal stem cells,
liver reserve cells, neural stem cells, pancreatic stem cells and/or
embryonic stem cells. Additionally, the cells may be genetically
modified. A three-dimensional tissue construct is preferred. Once the
cell medium of the invention is conditioned, it may be used in any state.
Physical embodiments of the conditioned medium include, but are not
limited to, liquid or solid, frozen, lyophilized or dried into a powder.
Additionally, the medium is formulated with a pharmaceutically acceptable
carrier as a vehicle for internal administration, applied directly to a
food item or product, formulated with a salve or ointment for topical
applications, or, for example, made into or added to surgical glue to
accelerate healing of sutures following invasive procedures. Also, the
medium may be further processed to concentrate or reduce one or more
factors or components contained within the medium.