The present invention is related to a method and system for optimization of channel estimation and synchronization in an Orthogonal Frequency Division Multiplexing (OFDM) Multiple-Input Multiple-Output (MIMO) wireless communication system. In accordance with the present invention, all of the training sequences are simply constructed based on a basic code by cyclically shifting the basic code. The training sequences are transmitted from different antennas in parallel without performing inverse fast Fourier transform. As a result, there is no peak-to-average ratio problem. Channel estimation is performed in each receiver based on the samples before fast Fourier transform and the maximum-likelihood estimate of channel response in time domain is then mapped into the frequency domain. The channel estimation is not only very simple in implementation, but also very efficient in computation.