This invention discloses a substantially pure population of human Mullerian duct-derived epithelial cells and methods of isolating and culturing the Mullerian duct-derived epithelial cells. By carefully manipulating the microenvironment in which the Mullerian duct-derived epithelial cells are grown, multiple passages are attainable wherein the Mullerian duct-derived epithelial cells are capable of becoming uterine, cervical, vaginal, and oviductal cells. In addition, several uses of human Mullerian duct-derived epithelial cells and cells differentiating therefrom are disclosed herein.