A device, method and kit for collection of a urine specimen from an animal includes a cup to receive and hold the urine specimen, a lid to close cup after collection of the specimen, and a holder with a generally upright center extension member, a handle connected at the upper end of the extension member, and a cup holder extending forwardly from the lower end of the extension member and having an opening slidably receiving the cup for manual positioning of the cup under the animal from which the urine specimen is to be collected.