The present invention provides a lentivirus vector which comprises a coat protein containing VSV-G; a recombinant bird host which is constructed by infecting a bird host with the lentivirus vector; a method of constructing the same; a transgenic chimeric bird which is constructed by infection with the lentivirus vector and offspring thereof. The present invention also provides a method of constructing a G0 transgenic chimeric bird and offspring thereof which comprises infecting fertilized bird egg early embryos with a lentivirus vector comprising a coat protein containing VSV-G, and incubating the embryos; and a G0 transgenic chimeric bird and offspring thereof which is constructed by the method mentioned above. The present invention provides a transgenic bird which is constructed by infecting a bird early embryos with a lentivirus vector comprising a coat protein pseudotyped with VSV-G.

 
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> Device and Method to Facilitate Directed Delivery and Electroporation

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