The present invention relates to compositions and chromatography materials, such as materials for the separation of biomolecules. The composition can comprise granules comprising carbonaceous particles, and at least one carbonized substance selected from carbonized synthetic resins and carbonized pitches. The composition further comprises at least one organic group attached to the surface of the granules. Biomolecules to be separated can include proteins, viruses, and DNA plasmids. The composition can have granule sizes, particle sizes, and pore sizes designed for separation of a particular biomolecule depending on its size or binding properties.