The current invention provides methods to silence insect genes by using
unpackaged dsRNA or siRNA, in one embodiment such dsRNA or siRNA is
present in plant vascular tissue, preferably phloem, more particularly
phloem sap, and the insect is a plant sap-sucking insect. Also provided
are DNA sequences which when transcribed yield a double-stranded RNA
molecule capable of reducing the expression of an essential gene of a
plant sap-sucking insect, methods of using such DNA sequences and plants
or plant cells transformed with such DNA sequences. Also provided is the
use of cationic oligopeptides that facilitate the entry of dsRNA or siRNA
molecules in insect cells, such as plant sap-sucking insect cells.