Site-specific recombinases provide a means of efficiently manipulating
chromosomal sequences in mammalian cells in culture and in animals.
Embryonic stem cells containing recombinase nucleic acid constructs that
were expressed in the male germline simplify current protocols for
producing mice bearing homologously recombined alleles that have been
secondarily rearranged by a site-specific recombinase. In different
ProCre strains, between 80% and 100% of the progeny that inherited a Cre
target nucleic acid construct from males that were also heterozygous for
a ProCre nucleic acid construct inherited the Cre-recombined target.
ProCre nucleic acid constructs and recombined targets segregated in the
first generation. When ES cells prepared from one ProCre line were
transfected with vectors containing a loxP-flanked neomycin cassette,
G418 resistant, homologously recombined clones, in which the loxP sites
remained functional, were readily isolated.