Disclosed are a Nelumbinis Semen extract for treating depression, method of preparing the extract, and a pharmaceutical composition and a health food, comprising the extract. The Nelumbinis Semen extract is prepared by extracting Nelumbinis Semen with an alcohol or an alcohol solution. The Nelumbinis Semen extract has been demostrated using animal behavioral, bio-chemical and molecular biological methods to have strong antidepressive activity, as well as inhibiting immune suppression caused by depression, leading to normal immune responses, and reducing side effects of conventional antidepressants, thereby ensuring safety. Thus, The Nelumbinis Semen extract is useful for preparing a composition or health food for treating depression.