The present invention relates to anticancer activity against
hepatocellular carcinoma of an extract and fraction isolated from flowers
of Butea monosperma. Particularly, this invention relates to anticancer
activity against hepatocellular carcinoma of a composition containing
markered flavonoid glycosides such as butrin and isobutrin in the range
of 2 to 9% by weight, isolated from the flowers of Butea monosperma by
extracting the flowers with polar solvent like ethanol, methanol, aqueous
ethanol or water, removing fatty non-polar constituents by triturating
the extract with solvents such as ethylene chloride, methylene chloride,
chloroform or ethyl acetate, suspending the residue in water, extracting
with n-butanol and freeze drying the aqueous part.