A musical instrument stand with a neck lock assembly for a guitar-shaped musical instrument has a post, multiple legs and a neck lock assembly. The neck lock assembly is attached to the post and has a collar, a crossbar assembly and a crossbar fastener. The collar is attached securely to the post and has two distal ends. The crossbar assembly is mounted on one of the distal ends of the collar and has a crossbar pivotally mounted on the distal end of the collar and having a locking recess. The crossbar fastener is mounted on the other distal end of the collar and has two head-halves defining a tapered head. The crossbar pivots toward the crossbar fastener with the tapered head engaging the locking recess to securely lock the neck.