A fuel cell hose which is superior in flexibility (low-temperature flexibility) and in barrier performance against hydrogen gas or water without causing a defect such as buckling or cracking at the time of the press-fitting of a connector. The fuel cell hose comprises an inner layer in contact with fluid, a barrier layer formed on an outer peripheral surface of the inner layer and an outer layer formed on an outer peripheral surface of the barrier layer. The inner layer is made of a material (A) containing a polyolefin resin and a styrene-isobutylene block copolymer as essential components, the barrier layer is made of a material (B) containing an ethylene-vinyl alcohol copolymer and a modified polyolefin resin as essential components and the mixing ratio by volume of the ethylene-vinyl alcohol copolymer to the modified polyolefin resin is in the range of 95/5 to 30/70, and the outer layer is made of a material (C) containing a polyamide resin as an essential component. The barrier layer has an island-sea structure in which island phases composed of the modified polyolefin resin are dispersed in a sea phase composed of the ethylene-vinyl alcohol copolymer.