The present invention relates to isolated male tissue-preferred regulatory
sequences from Ms45 gene. The invention also relates to the use of Ms45
gene male tissue-preferred regulatory sequences in mediating male
fertility in a plant, and in producing hybrid seed. The Ms45 male
tissue-preferred regulatory region can be operably linked to exogenous
genes encoding cytotoxins or inhibitory molecules. The invention also
relates to plant cells, plant tissue and plants that contain the MS45
gene regulatory sequences.