A support for an array of fluorescently labeled samples comprises a
transparent body defining: (a) an array-support surface and (b) under
the support surface, in spaced apart relationship thereto, a field of
embedded optical features exposed to be illuminated by a broad light beam
of excitation radiation addressed to the support from a predetermined
general direction selected to produce a surface wave effect at the
support surface, the field of embedded optical features and the support
being so constructed that light of the beam incident on the features is
launched through the support at an angle to the support surface that
produces the surface wave effect of radiation in the manner that it can
produce fluorescence from the labeled samples to be imaged beyond the
support from a direction different from the direction of the
illumination. Fine transmissive and reflective features having surfaces
generally normal to radiation substantially at the critical angle, and a
grating illuminated at a non-normal surface are shown. A data acquision
system employing an elastic rotary motion reducer driven by a stepper
motor, under computer control, directs a broad illumination beam through
a series of small angular increments, an image is taken at each increment
by a CCD camera, and based upon energy references on the array-support
surface, a quilt image is formed, based on responses of the energy
references associated with localized regions of each image.