The invention relates to the herbal extract having anti-viral activity. More specifically, it relates to the herbal extract produced bv extracting the comminuted fruit of Fructus Ligustri Lucidi (privet fruit), Rhizoma Polygonati (sealwort) Herba Agrimoniae (agrimonia) Radix Rehmanniae Glutinosae Conquitae (steamed glutinous rehmannia) or the mixture thereof, with a low polar solvent, and to the method for in vitro antagonizing virus by contacting the herbal extract with viruses.