The invention relates to a reactor for cell and tissue culture particularly suited to the culture of cells or tissues in the form of thin layers, and comprises the means for ensuring a physiological stimulation and supply of nutrients in culture medium adapted to the reduced thickness of the culture. The reactor can be used for the preparation of an implant and also includes the means of ensuring a mechanical stimulation of tissues or cells and supplying nutrients in culture medium suitable for structural tissues. The reactor also comprises, in an advantageous way, the means or a form that allows easy introduction of the tissue or the solid part of an implant into the culture chamber and, for example, a wall or an elastically deformable element. In addition, the reactor can include means of temperature regulation.The reactor can be used for the culture of different types of cells and the preparation of implants in a variety of forms, compositions and applications.