We disclose a PS4 variant polypeptide derivable from a parent polypeptide,
the parent polypeptide having non-maltogenic exoamylase activity, which
PS4 variant polypeptide comprises one or more of the following
substitutions: G69P, A141P, G223A, A268P, G313P, S399P and G400P, with
reference to the position numbering of a Pseudomonas saccharophilia
exoamylase sequence shown as SEQ ID NO: 1. Such PS4 variant polypeptides
may be used as exo-amylases, particularly as non-maltogenic exoamylases.
Combinations of such PS4 variant polypeptides together with Novamyl are
disclosed.