A U-turn signal assembly for indicating to other drivers and pedestrians that a vehicle is making a U-turn. The vehicle has a left front quarter panel, a rear window, and a turn signal handle. The U-turn signal assembly has two light assemblies, one installed on the left front quarter panel of the vehicle and the other one installed on the rear window of the vehicle. The light assemblies each have a lens, backlit by a light fixture. The lens has a plurality of lettering and arrows for uniquely signaling the U-turn. An activation switch is attached to the turn signal handle. A control panel is in communication with the activation switch and light assemblies for controlling the light assemblies.