Polynucleotide constructs encoding EPSPS enzymes are disclosed. The constructs have at least one transcriptional enhancer operably linked to a soybean or Brassica promoter and to a sequence respectively encoding the soybean or Brassica EPSPS chloroplast transit peptide and soybean or Brassica EPSPS enzyme. The EPSPS enzymes comprise a modified region that confers herbicide resistance.