A bottle manipulation device facilitates one-handed manipulation and pouring from a bottle. The device has a bottle engaging member with a U-shaped fork having first and second branches on opposite sides of a bisecting plane for engaging on first and second opposite sides of a neck of a bottle respectively. A hook is pivotally connected to the bottle engaging portion for movement between a disengaged position with a blocking portion of the hook spaced from the bisecting plane and an engaged position with the blocking portion of the hook passing through the bisecting plane and blocking a front of the neck of the bottle for secure engagement of the bottle with the device. A method of using the device provides for secure manipulation and pouring.

 
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> Assay for phosphodiesterase function

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