A method for extraction of heterologous protein from monocotyledonous
plant seed comprises extracting the germ portion of the seed and
extracting and purifying the protein from the germ. Enhanced expression
in the germ is provided, and allows for improved efficiency in
production, and cost savings. Directing expression to the germ portion
further increases expression levels of the protein. The ubiquitin
promoter preferentially directs expression to the germ portion of plant
seed.