A purified mammalian proteoglycan, and genetic information encoding such
proteoglycans, having a core polypetide molecular weight of about 30 kD
to about 35 kD, and comprising a hydrophilic amino terminal extracellular
region, a hydrophilic carboxy terminal cytoplasmic region, a
transmembrane hydrophobic region between said cytoplasmic and
extracellular regions, a protease susceptible cleavage sequence
extracellularly adjacent the transmembrane region of the peptide, and at
least one glycosylation site for attachment of a heparan sulfate chain to
said extracellular region, said glycosylation site comprising a heparan
sulfate attachment sequence represented by a formula
Xac-Z-Ser-Gly-Ser-Gly (SEQ ID NO: 44), where Xac represents an amino acid
residue having an acidic sidechain, and Z represents from 1 to 10 amino
acid residues. Additional peptides having this glycosylation site and
genetic information useful for preparing a number of variations based on
this glycosylation site are also provided.