A method and apparatus for automated cell analysis of biological specimens
automatically scans at a low magnification to acquire images (288) which
are analyzed to determine candidate cell objects of interest. The low
magnification images are converted from a first color space to a second
color space (290). The color space converted image is then low pass
filtered (292) and compared to a threshold (294) to remove artifacts and
background objects from the candidate object of interest pixels of the
color converted image. The candidate object of interest pixels are
morphologically processed (296) to group candidate object of interest
pixels together into groups which are compared to blob parameters (298)
to identify candidate objects of interest which correspond to cells or
other structures relevant to medical diagnosis of the biological
specimen. The location coordinates of the objects of interest are stored
and additional images of the candidate cell objects are acquired at high
magnification. The high magnification images are analyzed in the same
manner as the low magnification images to confirm the candidate objects
of interest which are objects of interest. A high magnification image of
each confirmed object of interest is stored for later review and
evaluation by a pathologist.