A bicycle drive unit comprises a frame main unit, first and second cover members, a rear hub, a rear gear shift apparatus, an electric driving mechanism and a power supply unit. The frame main unit diverges from a front end portion toward first and second rear end portions with the front end portion movably coupled to a frame of a bicycle, and the first and second rear end portions coupled to the rear hub. The first and second cover members are coupled to transverse outer surfaces of the frame main unit such that a first space is formed between the first cover member and the frame main unit and a second space is formed between the second cover member and the frame main unit. The electric driving mechanism is disposed in the first space and linked to the rear gear shift apparatus.