The present invention provides predominantly callus-specific promoters obtainable from Brassica napus plants. According to the present invention there is provided a DNA fragment harbouring a callus specific promoter, said DNA fragment being present in clone pJB1178-21 or clone pJB1178-43, respectively deposited with the Centraal Bureau of Schimmelcultures (Baarn, the Netherlands) on 6 Feb. 2001 under no. CBS 109271 and no. CBS 109273. The DNA fragment according to the present invention is further characterised in that it comprises the nucleotide sequence represented by SEQ ID NO: 1 or SEQ ID NO: 2 or the full length sequences as depicted in SEQ ID NO: 8 or SEQ ID NO: 9 respectively or parts thereof. Further, the invention comprises the homologue sequences in Arabidopsis thaliana as depicted in SEQ ID NO: 10 and SEQ ID NO: 11, respectively.