A truncated form of the full-length, immunosuppression-inducing molecule,
CD200, CD200.sub.tr, was expressed in CHO cells and the transduced cells
used to produce mAbs to CD200.sub.tr. These mAbs detect simultaneous
expression of full-length CD200 and CD200.sub.tr in LPS-stimulated
dendritic cells (DCs). Moreover, CHO cells and DCs expressing
CD200.sub.tr as well as soluble CD200.sub.tr are able to inhibit
suppression of mixed leucocyte reactions in vitro which follows addition
of the soluble form of CD200 (CD200:Fc) in culture, demonstrating that
CD200.sub.tr is a physiological antagonist of CD200.