Isolated polynucleotide molecules provide recombinant PIV genomes and
antigenomes for production of recombinant PIV vaccines. The recombinant
genome or antigenome can be expressed with a nucleoprotein (N),
phosphoprotein (P), and a large (L) polymerase protein to produce
isolated infectious PIV particles. The recombinant PIV genome and
antigenome can be modified to produce desired changes, for example to
incorporate attenuating mutations from biologically derived PIV mutants
or to create chimeric PIV clones, to generate attenuated, immunogenic
viruses for vaccine use.