cDNAs (RISBZ1, RISBZ4, and RISBZ5) encoding bZIP transcription factors
were isolated from a cDNA library originating in rice plant seed. The
cDNAs encode novel proteins and have binding activity to the GCN4 motif.
Among them, RISBZ1 activated transcription mediated by the GCN4 motif by
100-fold or more. Since the expression of RISBZ1 precedes the expression
of a seed storage protein gene and is expressed only in maturing seeds,
it is suggested that RISBZ1 controls the expression of rice seed storage
proteins. In addition, by linking the recognition sequence of the
transcription factor, the GCN4 motif, in tandem and introducing it into
the promoter for a gene encoding seed storage protein to facilitate its
binding to the transcription factor RISBZ1, expression of a foreign gene
under the control of the modified promoters is greatly enhanced.