The present invention provides a method for preparation of 2'-deoxy-2',2'-difluoro-.beta.-cytidine or pharmaceutically acceptable salt thereof, comprising starting from 1,6-anhydro-.beta.-D-glucose as raw material, oxidizing, and fluorinating to obtain 2-deoxy-2,2-difluoro-D-ribofuranose as intermediate. The 2'-deoxy-2',2'-difluoro-.beta.-cytidine was finally prepared from the intermediate of 2-deoxy-2,2-difluoro-D-ribofuranose. The method is simple in operation and has a high yield. The method can effectively be used in large-scale production.