A system for verifying a layout of circuit traces on a motherboard includes a computer (1) and a database (2). The database is used for storing data generated and used by the system. The computer includes: a substandard layout area creating module (101) for creating substandard layout areas; a substandard segment data obtaining module (102) for obtaining from the database actual layout data on substandard segments placed in the substandard areas; a substandard length calculating module (103) for calculating a total length of all the substandard segments of a trace; and a satisfactory trace determining module (104) for comparing the total length of the substandard segments of the trace with a preset standard length for the trace, and determining whether the trace is satisfactory. A related method is also disclosed.