Devices and methods for assaying a sample for the presence of at least one analyte are provided. The subject compression devices include a base and a cover configured to apply a compression force to a structure comprising a first substrate separated from a second substrate by a separator when present in the device. The subject methods include contacting a sample with a first surface of a first substrate to produce a substrate supported sample, placing the substrate supported sample in contact with a second substrate to form a structure that includes the first and second substrates spaced-apart from each other by a separator, wherein one of the substrates is an array substrate having at least one array, applying a compression force to compress the structure together using a compression device and reading the at least one array to obtain a result.