The present invention relates to enzyme preparations consisting
essentially of an enzyme which has cellulytic activity and comprises a
first amino acid sequence having the following sequence
TABLE-US-00001
(SEQ ID NO: 79)
Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa Trp Xaa
1 2 3 4 5 6 7 8 9 10 11 12 13 14
and a second amino acid sequence having the following sequence
TABLE-US-00002
Trp Cys Cys Xaa Cys (SEQ ID NO: 80)
1 2 3 4 5
wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr
or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr
or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys
or His; at positions 9, 10, 12 and 14, respectively, of the first
sequence, and at position 4 of the second sequence, the amino acid is any
of the 20 naturally occurring amino acid residues with the provisos that,
in the first amino acid sequence, (i) when the amino residue at position
12 is Ser, then the amino acid residue at position 14 is not Ser, and
(ii) when the amino residue at position 12 is Gly, then the amino acid
residue at position 14 is not Ala, performs very well in industrial
applications such as laundry compositions, for biopolishing of newly
manufactured textiles, for providing an abraded look of cellulosic fabric
or garment, and for treatment of paper pulp. Further, the invention
relates to DNA constructs encoding such enzymes, a method for providing a
gene encoding for such enzymes, a method of producing the enzymes, enzyme
preparations containing such enzymes, and the use of these enzymes for a
number of industrial applications.