Blood separation media include a non-woven web of fibrillated and non-fibrillated synthetic staple fibers. The fibrillated synthetic staple fibers will most preferably have a Canadian Standard Freeness (CSF) of less than about 300 mL, whereas the non-fibrillated synthetic staple fibers have a CSF of greater than about 700 mL. Preferably, the fibrillated synthetic staple fibers are present in an amount between about 20 to 90 wt. %, and the non-fibrillated synthetic staple fibers are present in an amount between about 10 to about 80 wt. %. The fibrillated and non-fibrillated synthetic staple fibers may be formed of the same, or different, polymeric material, such as polyesters, polypropylenes, polyethylenes, poyacrylonitriles, and polyamides. Especially preferred blood separation media will comprise a blend of fibrillated acrylic fibers, and a mixture of non-fibrillated nylon and polyester fibers, wherein the ratio of nylon and polyester fibers in the mixture thereof is between about 1:4 to about 1:2. The blood separation media of this invention can be formed by conventional "wet-laid" processes. In use, a quantity of whole blood may be brought into contact with the blood separation media for a time sufficient to separate the erythrocytes therein.