The invention provides for a PS4 variant polypeptide derivable from a
parent polypeptide having amylase activity which may be selected from the
group consisting of: (a) a polypeptide comprising an amino acid mutation
at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223,
229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino
acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157,
178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising
an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146,
157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a
polypeptide comprising an amino acid mutation at each of positions 3, 33,
34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309
and 334; with reference to the position numbering of a Pseudomonas
saccharophilia exoamylase sequence shown as SEQ ID NO: 1, uses of such a
polypeptide as a food or feed additive, and nucleic acids encoding such.